Articles Scientifiques

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Setting up a diagram process for the elaboration of a new plant-based beverage from Pinus halepensis seeds: Selection of unit operations and their conditions
(journal of food process engineering_WILEY, 2021-11-28) Amina Abbou ; Nabil Kadri ; Adrien Servent ; Julien Ricci ; Khodir Madani ; Manuel Dornier ; Antoine Collignan et Nawel Achir
Aleppo pine seeds are a potential candidate for the production of nondairy milk due to their high protein (26%) and lipid (37%) content and their availability. For the first time, this study presents the implementation of a manufacturing scheme from seeds to final product, including pre-extraction, extraction, and post-extraction (physical and microbi-ological stabilization), using a 2(7-4)fractional factorial screening design. The seven fac-tors were seed soaking time, grinding time, temperature and seed/water ratio during extraction, xanthan gum concentration, homogenization pressure, and pasteurization temperature. Beverage quality analysis consisted of measurements of chemical compo-sition, color, stability, and rheological behavior. The results of experimental design showed that the dry matter as well as the lipid and protein contents increased signifi-cantly by increasing the ratio (grain/water) and the grinding time. On the other hand, the extraction temperature led to a better extraction of lipids but a lower extraction of proteins. Regarding stability, xanthan gum had a negative effect, while homogenization pressure and pasteurization temperature showed their effectiveness in decreasing the particle size and increasing the physical stability of the product. Practical applications Plant-based drinks, also called plant milks, are interesting alternatives to animal milk.They have similar organoleptic properties but are more sustainable and offer very dif- ferent nutritional composition depending on the plant used. As the consumers demand is constantly increasing, setting up fabrication processes from new plant sources is relevant. Aleppo pine seeds are a potential matrix for the production of vegetable beverages because they are very nutritious and available in the entire Mediterranean area. This study presents the setting up of a fabrication diagram of a plant-based beverage made from Aleppo pine seeds. Seven production parameters were tested, including pre-extraction (seed conditioning), extraction, and post-extraction (physical and microbiological stabilization), thanks to a fractional factorial 2(7-4)screening design. The best conditions enabled the production of a final bever-age comparable to other commercialized plant based beverages in terms of nutri-tional and organoleptic quality.
Efficient Adsorption of Chlortetracycline and Ibuprofen Onto Mg–Al Layered Double Hydroxide: Equilibrium, Kinetic, and Thermodynamic Studies
(chemeistrySelect, 2026) Kheira Addouch, Hakima Cherifi, Soumia Seddari, Radhia Yous, Nasma Bouchelkia, Lotfi Mouni
Magnesium–aluminum layered double hydroxide (Mg─Al LDH) with a Mg/Al molar ratio of 2:1 was synthesized by a simple co-precipitation method and evaluated for the simultaneous removal of chlortetracycline (CTC) and ibuprofen (IBP) from aqueous solutions. SEM–EDX, FTIR, and XRD analyses confirmed the successful formation of a well-defined layered structure with a basal spacing of 7.65 Å and a pHpzc of 6.15. Batch adsorption experiments showed rapid equilibrium within 30 min for CTC and 60 min for IBP, with removal efficiencies reaching 99% under optimal conditions (293 K, adsorbent dosage 2 g L−1, initial concentration 50 mg L−1, pH 7). The adsorption kinetics followed the pseudo-second-order model for CTC and the pseudo-first-order model for IBP, while equilibrium data were best described by the Langmuir isotherm, yielding maximum adsorption capacities of 121.61 mg g−1 for CTC and 100.41 mg g−1 for IBP. Thermodynamic parameters indicated spontaneous and exothermic adsorption, with stronger interactions for CTC. The uncalcined Mg─Al LDH exhibited adsorption performance comparable to or exceeding that of many modified LDH-based materials, highlighting its potential as a simple, cost-effective, and sustainable adsorbent for pharmaceutical wastewater treatment.
Evaluation of the Antioxidant Potential and Selective Cytotoxicity of Fumaria agraria Alkaloids Against Human Breast Cancer Cells
(Pharmacognosy Magazine, 2026) Khalida Bougoffa-Sadaoui,Sid Ali Zaidi,Nouara Djouadi,Massilia Kaci,and Halima Ouadid-Ahidouch
Aim/Background This study aimed to investigate the antioxidant capacity, hemolytic effect, and cytotoxic potential of the alkaloid fraction isolated from Fumaria agraria, with an emphasis on its possible use as a therapeutic agent against cancer cells.
Study of bioactive volatile compounds from different parts of Pistacia lentiscus L. extracts and their antioxidant and antibacterial activities for new active packaging application.
(Food Control_ELSEVIER, 2020-07-28) Sabrina Djebari ; Magdalena Wrona ; Asma Boudria ; Jesús Salafranca ; Cristina Nerin ; Kenza Bedjaoui et Khodir Madani
Macerates of fruits and leaves of Pistacia lentiscus L. were prepared and analysed with the aim of applying them for active packaging. The profile of forty-four different bioactive volatile compounds was obtained by means of gas chromatography-mass spectrometry and solid-phase microextraction gas chromatography-mass spectrometry. Antioxidant capacity was evaluated by three different methods (2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid and reducing power) which confirmed stronger antioxidant properties in case of leaves macerate. Total phenolic and flavonoids content was determined and showed that macerate leaves presented 15 times more phenolic compounds and 20 times more flavonoids than macerate fruit. Moreover, the analysis of antimicrobial properties of macerate leaves in comparison with macerate fruits revealed very strong antimicrobial properties. Finally, macerate leaves extract was incorporated in an adhesive and a new active multilayer packaging was designed, and its antioxidant capacity as free radical scavenger was confirmed by a method based on in situ hydroxyl radicals generator.
Jam processing: Effect of pectin replacement by locust bean gum on its characteristics
(Mediterranean Journal of Nutrition and Metabolism_IOS Press, 2020-11-28) Aida Mekhoukhe ; Nacer Mohellebi ; Tayeb Mohellebi ; Leila Deﬂaoui-Abdelfettah ; Sonia Medouni-Adrar ; Lila Boulekbache-Makhlouf et Khodir Madani
OBJECTIVE : the present work proposed to extract Locust Bean Gum (LBG) from Algerian carob fruits, evaluate physic-ochemical and rheological properties (solubility). It aimed also to develop different formulations of strawberry jams with a mixture of LBG and pectin in order to obtain a product with a high sensory acceptance. METHODS : the physicochemical characteristics of LBG were assessed. The impact of temperature on solubility was also studied. The physical and the sensory proﬁle and acceptance of ﬁve Jams were evaluated. RESULTS : composition results revealed that LBG presented a high level of carbohydrate but low concentrations of fat and ash. The LBG was partially cold-water-soluble ( ∼ 62% at 25◦C) and needed heating to reach a higher solubility value ( ∼ 89%at 80◦C). Overall, the sensorial acceptances decreased in jams J3 which was formulated with 100% pectin and commercial one (J5). The external preference map explained that most consumers were located to the right side of the map providing evidence that most samples appreciated were J4 and J2 (rate of 80– 100%). CONCLUSION : In this investigation, the LBG was used successfully in the strawberry jam’s formulation.
The petroleum-degrading bacteria Alcaligenes aquatilis strain YGD 2906 as a potential source of lipopeptide biosurfactant
(Fuel_ELSEVIER, 2020-09-08) Drifa Yalaoui-Guellal ; Samira Fella-Temzi ; Salima Djafri-Dib ; Sunil Kumar Sahu ; Victor U. Irorere ; Ibrahim M. Banat et Khodir Madani
Soummam river sediments were used to isolate a biosurfactant-producing and petroleum-degrading bacterium. The strain was identified as Alcaligenes aquatilis YGD 2906 using phenotypic characterization and 16S ribosomal RNA sequencing. The culture supernatant of the isolated strain showed no haemolytic activity had an oiled displacement of 23.66 ± 0.57 mm and Emulsification index (E24) of 68.5% ± 0.5%. The biosurfactant produced in the minimal medium was extracted by acid precipitation and quantified gravimetrically, with a yield of 4.2 ± 0.01 g/L. The crude Biosurfactant was determinate by TLC and MALDI-TOF-MS as a lipopeptide with protein and lipid content of 8.49 ± 0.19% and 52.66 ± 1.16% respectively. This lipopeptide structure was confirmed by HPLC-MS/MS. This technique gave two main peak ranges which are typical of surfactins, iturins and fengycin. Tandem mass spectrometry was further used to elucidate the structure of the lipopeptide produced by the strain. The non-haemolytic nature of the lipopeptide produced by this strain offers opportunities for biomedical applications. Further work is needed to optimize production and analyze potential biomedical uses of this lipopeptide including antimicrobial properties.
Convective and microwave drying of coriander leaves: Kinetics characteristics and modeling, phenolic contents, antioxidant activity, and principal component analysis
(Journal of Food Process Engineering_WILEY, 2021-11-23) Khokha Mouhoubi ; Lila Boulekbache-Makhlouf ; Wafa Mehaba ; Hayat Himed-Idir et Khodir Madani
In this study, coriander leaves were subjected to two different drying techniques: con- vective (40, 80, and 120 C) and microwave (100, 500, and 1,000 W). The effects of both processes on the kinetic parameters variation, modeling, and phytochemical qual- ity were studied. Kinetic parameters variation showed that time reduction (2.53 min), high drying rate (3.08 kg water/kg dm min), and high diffusivity (1.21 10 10m2/s) were obtained during microwave drying. For energy consumption, an opposite trend was observed between the variation in the specific electrical energy consumption (SEC e ), which is significant during convective drying (208.33 10+ 07MJ/kg H 2 O), and the energy efficiency (EE), which is obtained during microwave one(12.16 10 03 %). The kinetic data modeling done on 23 empirical models allowed us$to choose the Sledz model as the best describing the drying kinetics of coriandersince it presented better predictive performances with .9947 < adjusted R2< .9995,0.0001 < χ 2< 0.0005, and 0.0070 < RMSE<0.0228 values. In relation to the bioac-tive compounds, the highest values of the total phenolic content (TPC) and antioxi-dant activities (ABTs and DPPH) were observed during microwave drying with 0.37 mg GAE/mL, 83.33, and 71.94%, respectively. Correlations between kinetic parameters, energy consumption as well as phytochemical quality were established using principal component analysis (PCA). As a result, the innovative microwave pro-cess proved to be the best method for drying coriander leaves. Practical applications: The evolution of technological conservation processes has led to the development of a wide range of modern drying processes. The success of these processes can be explained by the many advantages attributed to them: shorter drying times, high drying rate, high energy efficiency, and product quality. The most difficult is the appropriate choice of a drying method with highly energy efficient and consumes less energy while maintaining the best quality. Fresh coriander leaves are widely used to flavor foods. However, they are highly perishable and require immediate processing or preservation. Thus, the objective of this study is to compare two drying processes, convective and microwave drying of coriander leaves based on kinetic data, modeling, calculation of moisture diffusivity and energy consumption, aswell as evaluation of the dried powders quality. The results obtained will be useful for the technological design of convective or microwave drying for coriander leaves preservation.
: POLLEN PROFILES, PHYSICOCHEMICAL CHARACTERISTICS, AND ANTIOXIDANT ACTIVITIES OF TWO HONEY SAMPLES FROM JIJEL CITY (ALGERIA)
(The Annals of the University Dunarea de Jos of Galati Fascicle VI – Food Technology_Galati University Press, 2021-07-28) RABHA AYAD ; NADIA AMESSIS-OUCHEMOUKH ; SALIM OUCHEMOUKH ; KHODIR MADANI et LILA BOULEKBACHE-MAKHLOUF
Honey is a sweet and flavorful animal product that comes from nectar and/or honeydew. It is used in different nutritional and therapeutic fields. This study aimed at the determination of the botanical origin, the physicochemical characteristics, and the antioxidant activities of two honey samples from two locations in Jijel City (Algeria). The analyzed honey complies with the standards of the Codex Alimentarius Commission. Pollen analysis showed that honey sample H1 was polyfloral and honey sample H2 was monofloral. Quality parameter analysis revealed that moisture content varied from 13.21 to 16.44%. The average pH was 4.34, and the electrical conductivity was 0.56 mS/cm. Protein, proline, and HMF contents were 32.08 mg EBSA/ 100 g, 435.47 mg/kg, and 3.49 mg/kg, respectively. Both analyzed kinds of honey were levorotatory. Phenolic compounds and flavonoids were found to be higher in honey H1 with 60.93 mg of EAG/100g and 20.92 mg of EC/100g, respectively. Results of antioxidant activities showed that honey H1 was much more effective in reducing iron and copper than honey H2 and gave the best total antioxidant capacity. In addition, the study of the antiradical activity against DPPH and ABTS revealed that honey H1 was able to scavenge DPPH and ABTS radicals with 48.88 and 18.21 %, respectively.
Comparative mapping approach of energy intake and expenditure of obesity prevalence among school adolescents
(Clinical Nutrition ESPEN_ ELSIVIER, 2021-04-07) Fouzia Yous; Salim Zaabar; Omar Aoun; Nawel Adjeroud-Abdellatif et Khodir Madani
Objective: This study aims to describe the prevalence of overweight and obesity in an obesogenic environment among adolescents in the wilaya of Bejaia (eastern Algeria), and to assess their Physical Activity Level (PAL), Daily Energy Expenditure (DEE) and Total Energy Intake (TEI). Methods: A cross-sectional study was carried out on a sample of 3038 adolescents consisting of 1635 girls and 1403 boys in middle and high schools in 19 regions of Bejaia. The average age was 14.78 ± 2.53. Two questionnaires were assigned to collect data on their nutritional habits (24-h dietary call) and physical activity levels. BMI measurement was adopted in this study to determine the weight status of this sample and to assess the distribution of BMI, PAL, DEE, TEI over geographical areas of an administrative territory using the Student test descriptive statistical method (p < 0.05). Results: The prevalence of obesity and overweight was equal to 3.3%, the physical activity level in adolescents was 1.72 ± 0.21, with boys more likely to participate in sports than girls (16.5% for boys and 6% for girls). However, energy intake (3775.44 ± 719.07 kcal/day), energy expenditure (3144.13 ± 850.54 kcal) and body mass index (30.51 ± 2.99 kg/m2 ) were high. Body mass index (BMI) is negatively correlated with PAL (r ¼0.05) and positively correlated with TEI (r ¼ 0.31) and DEE (r ¼ 0.30), these results show that DEE and TEI factors play an important role in the prevalence of BMI with excessive consumption of high carbohydrate (614.55 g) and fat (98.9 g) foods, which is due to poor diet and are related to insufficient level of physical activity. The mapping shows that there is a disparity between the studied parameters: high BMI, low PAL and consumption of unhealthy foods (DEE, TEI). Conclusion: The lack of physical activity and the imbalance between energy intake and expenditure are the main factors that could lead to an increased risk of obesity in adolescents
Microwave-assisted hydrodistillation of essential oil from fennel seeds: Optimization using Plackett–Burman design and response surface methodology
(Journal of Applied Research on Medicinal and Aromatic Plants_ ELSEVIER, 2021-04-05) Hayet Boudraa ; Nabil Kadri ; Lotfi Mouni et Khodir Madani
The study aims to optimize the microwave-assisted hydrodistillation (MAHD) of Foeniculum vulgare seed essential oil (FSEO), for the purposes of maximizing efficiency and reducing costs in terms of time and energy. Plackett–Burman design (PBD) was investigated to screen the six different variables affecting FSEO extraction and select the most significant ones. Response Surface Methodology (RSM) was used to evaluate the interactions of the significant parameters and to determine optimal conditions. MAHD and the traditional hydrodistillation (HD) techniques were compared in terms of extraction efficiency, economic and environmental impacts, and chemical composition of the FSEO. The optimum conditions of the studied responses were particle size less than 333.4673 μm, at the irradiation time of 20 min and a microwave power of 305.9282 W with a solid-liquid ratio of 0.1477 g/mL. Gas Chromatography (GC) analysis showed that the FSEO composition in MAHD and HD is similar with slight differences. Therefore, the results show that the microwave-assisted hydrodistillation method is an innovative, economical and environmentally friendly approach to efficiently and safely extract essential oil an economic and green innovative approach to extract efficiently and safely the essential oil (EO).
Phenolic compounds from an Algerian medicinal plant (Pallenis spinosa): simulated gastrointestinal digestion, characterization, biological and enzymatic activities
(Food & Function_Royal Society of Chemistry, 2021-02-07) Hanane Amrani-Allalou ; Lila Boulekbache-Makhlouf ; Luana Izzo ; Lynda Arkoub-Djermoune ; Mohamed Lamine Freidja ; Khokha Mouhoubi ; Khodir Madani et Gian Carlo Tenore
Pallenis spinosa is a medicinal plant which is used in folk medicine as curative or preventive remedies for various diseases. Individual phenolic compounds from the methanolic extracts of its flowers, leaves and stem were determined by high performance liquid chromatography method (HPLC) and total phenolic contents (TPC) was evaluated by Folin-Ciocalteu assay. The stability and bioactivity (antioxidant activity, micellar cholesterol solubility, α-amylase, and angiotensin converting enzymes (ACE) inhibitory effects) of these extracts in the gastrointestinal environment was determined before and after their protection hydroxypropylmethylcellulose (HPMC) capsules. HPLC analysis revealed the presence of thirteen phenolic compounds with nine flavonoids and four phenolic acids. Except for Kaempferol, the twelve other compounds have not previously detected in the aerial part of the studied plant. Quantification of phenolics by HPLC and Folin Ciocalteu methods revealed that the greatest TPC was detected in the flower extracts (104.31 ±0.80 and 145.73 ±0.48 mg EGA/g of extract, repectively). Leaves extracts displayed the best antioxidant capacity against the two tested radicals DPPH and ABTS (IC50=1.24 ±0.03 and 0.94 ±0.02 mg/mL, respectively), FRAP assay (IC50=0.50 ±0.02 mg/mL), α-amylase inhibitory (IC50=1.25 43 ±0.00 mg/mL) and angiotensin activity with an inhibitory percent of 30.10 ±0.12%. The best activity shown by stem extracts was against micellar cholesterol solubility (67.57 ±0.00%). A strong decrease in TPC and their bioactivity were observed after the gastrointestinal digestion (GID) in non encapsulated extracts. These results showed that P. spinonsa is a good source of phenolic compounds and the GID affects significantly their composition, content and bioactivity.
LC-DAD-ESI-MS/MS analysis and cytotoxic and antiproliferative effects of chlorogenic acid derivative rich extract from Nerium oleander L. pink flowers
(Food & Function_ Royal Society of Chemistry, 2021-03-31) Sheila Cristina Oliveira-Alves; Ana Teresa Serra; Khalef Lefsih; Madani Samah; Andreia Bento da Silva; Khodir Madani et Maria Rosário Bronze
This study compared antioxidant (DPPH, ABTS, FC) and anticarcinogenic (MTT test) 3 activities of three hydroethanolic extracts from different morphological parts of Nerium 4 oleander lin, (leaves (LE), pink (PFE) and white flowers (WFE).All of the extracts exhibited 5 different antioxidant and anticarcinogenic activities based on their antioxidant presence in the 6 extract. Principal component analysis (PCA) was applied to understand the relationship 7 between the antioxidant and anticarcinogenic activity, and to gain insight into the similarities 8 and differences between the three extracts. Basing on the set data, this analysis clearly 9 indicate that WFE have more potent antioxidant activity and a potential to inhibit HT29 10 colorectal cancer cell growth than LE and PFE respectively. The FTIR analysis indicated the 11 presence of various functional groups such as alcohol, phenol, alkane derivatives, aromatic 12 compound, that are responsible for the biological properties of studied extracts.
A sustainable valorization of industrial tomato seeds (cv Rio Grande): Sequential recovery of a valuable oil and optimized extraction of antioxidants by microwaves
(Journal of Food Processing and Preservation_ WILEY, 2021-10-19) Toufik Ouatmani; Hayate Haddadi- Guemghar; Lila Boulekbache- Makhlouf; Djamila Mehidi- Terki; Azzedine Maouche et Khodir Madani
Tomato seeds are a major waste of tomato paste manufacturing. In this study, an in- teresting yield of oil was obtained (21.69%) from the seeds of an industrial tomato by- product (Rio Grande variety), after a Soxhlet extraction. The fatty acid profile of the extracted oil was established by gas chromatography and the tomato seed oil (TSO) was rich in unsaturated fatty acids (79.83%). After that, microwave- assisted extrac- tion (MAE) was used to recover phenolic compounds from the defatted tomato seeds (DTS). The effect of three independent variables (microwave power, irradiation time, and solvent fraction) on the total phenolic content (TPC) and the antioxidant activity (AA) was estimated by using response surface methodology (RSM). The approach of desirability was applied to define the best operative conditions for the simultaneous extraction of TPC and AA which resulted in 700 W for microwave, 70 s for irradiation time, and water as extracting solvent. Practical applications By adopting a sustainable development approach, agro- industrial manufactories could earn the challenge of turning their waste into a source of additional income while also saving on disposal costs. In this study, a process by exhaustion following a smart and green approach was designed to recover added- value nutrients from an industrial tomato byproduct. First, tomato seeds were used as a feedstock to extract a multi- useful oil (edible, biofuel, cosmetic, etc.). Then, the residue was extracted again with hydro- ethanol as a safe and green solvent, by using microwaves as a green and cheap technology to optimize the extraction of antioxidants. In addition, com- putational tools (RSM and desirability approach) were applied for optimization and modeling of the operative conditions.
Effect of novel bottle inner surface coatings made with polylactic acid and plant extracts on the physiological status of Pseudomonas aeruginosa in real liquid foods using flow cytometry
(Packaging Technology and Science_WILEY, 2021-11-22) Saliha Oussaid ; Khodir Madani ; Karim Houali ; Ismael Marcet ; Tahar Amrouche ; Manuel Rendueles et Mario Diaz
Polylactic acid (PLA) was used, together with a novel optimized mixture (OM) of Thymus fontanesii essential oil (EO d ) and the ethyl acetate (EA) fraction from Scirpus holoschoenus extract, to develop antimicrobial coatings for the inner surface of bottles for the preservation of food liquids against the pathogenic bacterium Pseudomonas aeruginosa. The effect of these coatings on the physiological status of P. aeruginosa was tested by flow cytometry (FC), filling coated bottles with previously contaminated fish soup or egg whites. As was expected, the release of phenolic compounds (PCs) from the inner coatings of the bottles to the liquid was temperature dependent. Although their antimicrobial activity in contaminated egg whites was negligible, probably due to the neutralization of bioactive substances by ovalbumin, the PLA-OM-coated bottles had a high antimicrobial effect when they were filled with fish soup. In this latter case, the percentage of viable cells greatly decreased, and the percentage of dead cells, killed by membrane damage, increased.
Ultrasound and heat treatment effects on Staphylococcus aureus cell viability in orange juice
(Ultrasonics Sonochemistry _ELSEVIER, 2021-09-07) Akila Amir Tahi ; S´ergio Sousa ; Khodir Madani ; Cristina L.M. Silva et Fatima A. Miller
Ultrasounds are being considered an excellent alternative technology in juice preservation. Yet, when combined with heat treatment, the process seems to be further intensified. This work aimed to evaluate and compare the impact of ultrasounds and heat treatments, when applied alone or in combination, on Staphylococcus aureus survival in orange juice. Inoculated commercial pasteurized orange juice was treated at different times at 20, 30, 40, 50 and 60 ◦C. SEM analyses were applied to identify morphological changes in S. aureus cells appearance. The microbial inactivation data were fitted using two mathematical models, depending on the behaviour observed. Sonication at 20, 30, and 40 ◦C induced 4.02 ± 0.52, 3.80 ± 0.49 and 4.30 ± 0.74 log cycles reduction of S. aureus after treatments of 90, 60 and 60 min, respectively. The heat treatments at the same temperatures had no impact on S. aureus survival. When 50 and 60 ◦C were applied, more than 5-log reductions were attained for both thermosonication and heat treatments alone. A synergistic effect was observed between sonication and high temperatures. At 50 ◦C, the thermosonication reduced the treatment time from 60 to 35 min and the microbial load from 5.14 ± 0.08 to 10.76 ± 0.28 log cycles reduction, compared to heat treatment alone. Results from SEM images showed that cells undergo membrane damage during sonication exposure. This was observed by collapsed cells, cell disruption, and holes in the cell’s membrane. Thermosonication proved to be a viable alternative to thermal pasteurization of orange juice since milder treatments can be safely applied, improving the final product quality.
OPTIMIZATION OF THE MICROWAVE ASSISTED EXTRACTION AND BIOLOGICAL ACTIVITIES OF POLYPHENOLS FROM LEMON VERBENA LEAVES
(The Annals of the University Dunarea de Jos of Galati Fascicle VI – Food Technology_Galati University Press, 2021-02-02) ZOHRA TOUATI; MENANA GUEMGHAR; KENZA BEDJAOUI; NABILA DJERRADA1; KAHINA DJAOUD1 ; NAWEL ADJEROUD1; KHODIR MADANI et LILA BOULEKBACHEMAKHLOUF
The present study aims to optimize the extraction of phenolics by microwaveassisted extraction (MAE) using the response surface methodology (RSM), from Lemon verbena leaves. The optimized extract was tested for its antioxidant activity using two methods (DPPH and reducing power) and its antibacterial efficiency by using disk diffusion assay and broth microdilution, against two Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) and two Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633) strains. Under the optimized conditions (40% (v/v): of ethanol concentration, 188 s of irradiation time, 600 W of microwave power and 1:40 g/mL of solid-to-liquid ratio) the total phenolic content (TPC) was 67.87±1.61 mg GAE/g DW. The IC50 of the extract was 139.65±1.44 µg/mL and 56.60±2.79 µg/mL for DPPH inhibition and reducing power, respectively. The best antibacterial activity was shown by the extract obtained by MAE with lower MBC (1.56 to 18.75 mg/mL) and MBC/MIC ratio. Lemon verbena extract can be used as an ingredient in cosmetics, food supplements and herbal medicinal products due to its interesting biological properties.
Identification of some Opuntiaspp. from two Algerian regions and ultrasound assisted extraction of their phenolic compounds
(Journal of the Professional Association for Cactus Development_Professional Association for Cactus Development, 2021-06-29) Amokrane Mahdeb; Nawel Adjeroud-Abdellatif; Azzedine Mazari3; Liberato Portillo; Kamelia Ait Abdelouhab; Dounia Ait Maamer et Khodir Madani
Much attention has been paid to cacti for their distinctive characteristics. The nopal Opuntiaficus-indicaand relatedspecies are found inarid and semi-arid regions around the world. O.ficus-indica shows considerable variability in response to environmental conditions through variation of areola and spine attributes, among other traits. Therefore, the present study sought to morphologically identify some cactuspear plants in two areas of Algeria (Bejaia and Souk-Ahras)in orderto evaluate the cactus pears by optimizing the extraction conditions of the phenolic compounds of the cladode, to evaluate the antioxidant activity of these compounds,to measure the total flavonoid content,and to compare the yields among three Opuntiaspp. (O. ficus-indica, O. megacanthaand O. amyclae) from the sites within the study regions. These three Opuntiaspp. were subjected to extraction of polyphenols using a conventional method, and an ecofriendly process using ultrasound-assisted extraction(UAE)technique.Once the extractionswas completed, the total phenolic contentandflavonoids of the extracts were characterized. In addition, the antioxidant capacityof the total phenolic compounds (TPC)wasdetermined. The UAEwas better than conventional extraction for the recovery of TPC. The use of a solvent mixture (50% water/ethanol, v/v) improved the TPC extraction efficiency. Theseresults show thatUAE was a useful tool for the extraction of phytochemicals from cactuspear cladodes. Other factors and conditions that influence the optimization of TPC extractionshould also be studied, such as pH, ultrasound frequencyas well asecological factors that could possibly influence extraction efficiency
Effect of different compatibilization routes on the mechanical, thermal and rheological properties of polypropylene/cellulose nanocrystals nanocomposites
(Polymer Bulletin, 2024-03-21) Lilia Benchikh, Tahar Aouissi, Yazid Aitferhat, Hichem Chorfi, Ilyes Abacha, Maya Kebaili, Melia Guessoum, Abdelhafid Merzouki, Yves Grohens, Mauro Carraro & Souad Djellali
The combination of cellulose nanocrystals (CNCs) with synthetic polymers like polypropylene (PP) offers the opportunity to create advanced nanocomposites with significant advantages in terms of mechanical properties, improved thermal stability, enhanced barrier properties, and sustainability. However, a major drawback for incorporation of CNCs in polymer matrices is their poor dispersion and incompatibility with industrial processing of many composites. This work aims to improve the dispersion of hydrophilic CNCs in a hydrophobic matrix using a method which could be adapted for the industrial level. CNCs are extracted from Ampelodesmos mauritanicus (El Diss plant) (CNCD) and incorporated in a polypropylene matrix using the masterbatch method. A first nanocomposite (PP/CNC-Gr) was prepared by adding maleic anhydride (MA) to a CNCD/PP suspension, while the second nanocomposite (PP/CNC-Co) was achieved by using a MA-grafted PP (PP-g-MA) as a third component. Concentrated masterbatch underwent solution casting followed by homogenization in a Brabender mixer. Mechanical properties comparison showed that PP/CNC-Co nanocomposites exhibited greater resistance relatively to PP/CNC-Gr nanocomposites. Moreover, PP/CNC-Co nanocomposites revealed an improved thermal stability and a higher complex viscosity, particularly with 3% of CNCD. Properties enhancements are attributed to the reaction between MA groups grafted to PP chains and hydroxyl groups of CNCDs, which enables an improved interfacial adhesion, leading to more continuous materials, as perceived from the increase in viscosity and morphology observation. On the other hand, MA reaction with PP chains and CNCD induced only a partial coverage of CNCDs during nanocrystals treatment, conferring relatively lower properties to PP/CNC-Gr nanocomposites.
Response Surface Methodology: An Optimal Design for Maximising the Efficiency of Microwave-Assisted Extraction of Total Phenolic Compounds from Coriandrum sativum Leaves
(Process, 2024-05-19) Soraya Hihat ,Noureddine Touati, Abdelhakim Sellal and Khodir Madani
The optimization of total phenolic compounds (TPC) extraction yield and maximization of total antioxidant capacity (TAC) from coriander leaves were investigated using response surface methodology. The extraction of TPC was carried out using microwave-assisted extraction. A Box-Behnken design was used to study the effects of the three independent variables, solvent concentration (ethanol/water 20–80%), microwave power (100–500 watt) and irradiation time (30–150 s) on the response. A second-order polynomial model was used to predict the reaction. The regression analysis showed that about 99% of the variations could be explained by the models. The predicted values were 50.97 GAE/g dw and 5.75 mg GAE/g dw for TPC and TAC, respectively. The reaction surface analysis showed that the optimum extraction parameters that maximized the extraction of antioxidants yield were 52.62% ethanol, 452.12 watt and 150 s. Under optimal conditions, the experimental values for TPC and TAC were 49.63 ± 0.93 mg GAE/g dw and 5.55 ± 0.07 mg GAE/g dw, respectively. The experimental values are in agreement with the predicted values, indicating the suitability of the model used and the success of the response surface methodology in optimizing the extraction conditions.
Anti-inﬂammatory and prolonged protective effects of Artemisia herba-alba extracts via glutathione metabolism reinforcement
(South African Journal of Botany_ ELSEVIER, 2021-07-09) Nadjet Bouchara; Fran¸cois Senejoux; Didier Fraisse; Catherine Felgines; Florence Caldéﬁe-Chezet; Marie-Paule Vasson; Khodir Madani et Adrien Rossary
Artemisia herba-alba (AHA) is a medicinal plant known for its preventive capacities against chronic diseases. Inﬂammation is a naturally occurring cellular response to noxious stimuli, and both acute and chronic inﬂammation are involved in human disorders. Preventing and limiting the negative impact of inﬂammation by using phytocompounds has attracted a lot of attention. Here, we show that AHA extracts are potent anti- inﬂammatory products at low doses in white blood cells, treated with phorbol myristate acetate (PMA), a pro-inﬂammatory trigger. Regardless of the mode of extraction, i.e. maceration or microwave, all extracts reduced on dose-dependent manner reactive oxygen species (ROS) production induced by the pro-inﬂam- matory compound PMA in white blood cells and leukemic Jurkat cell line. The induction of the anti-inﬂam- matory effect was evaluated by the measurement of glutathione metabolism (glutathione reductase, transferase, and peroxidase), and protein-free thiols in Jurkat cells. Jurkat cells incubated with low to high concentrated AHA extracts, ranged from 10 to 100 g/mL, induced a fast cell response measured at one and six hours. Interestingly, extracts from the microwave showed a stronger induction, 5 to 10 fold changes at early time points, and maintained over 72 h. On the other hand, AHA extracts showed a moderate anti-prolif- erative effect in the leukemic Jurkat line. We concluded that AHA extracts enhance directly anti-inﬂamma- tory cell response, earlier via ROS induction. Later, however, vigorously maintained, independently from ROS, suggesting the intervention of the cellular metabolism in enhancing these effects. Considering the pre- ventive and anti-inﬂammatory potential of AHA, regular consumption is advisable, and phytotherapeutic applications are encouraged to treat local inﬂammations.

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