Articles Scientifiques

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Improving camphor solubility and stability through α-cyclodextrin encapsulation: characterization, cytotoxicity investigation, and bull sperm preservation evaluation
(Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2025-05-22) Hayet Ahlem Lezrag · Sofiane Fatmi · Lamia Taouzinet · Zahra Toutou · Katarzyna Pokajewicz · Farid Ait Merzeg · Mohamed Skiba · Piotr P. Wieczorek · Mokrane Iguerouada
Camphor, a crystalline white substance with diverse biological benefits, faces limitations in human health applications due to its volatile and hydrophobic nature, which impedes its solubility and stability. This study addresses these challenges by investigating the encapsulation of camphor within various cyclodextrins (CDs), particularly focusing on α-cyclodextrin (α-CD), to enhance its solubility and protect it from environmental degradation. The formation of camphor-CD inclusion complexes was confirmed through a combination of infrared spectroscopy (FTIR), X-ray diffraction (XRD), and ther mogravimetric analysis (TGA). Among the CDs tested, α-CD demonstrated the highest efficiency, achieving a solubility coefficient of 23.38 M⁻¹ and inclusion efficiencies of 24.37% and 34.63% for 1:1 and 1:2 stoichiometries, respectively. Biological evaluations revealed that the Camphor/α-CD complex exhibited significant preservative effects on sperm motil ity at low concentrations while demonstrating a toxic effect on red blood cells. Additionally, the complex showed promis ing radical scavenging activity as assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. These findings suggest that camphor, when complexed with α-CD, holds considerable potential for novel applications in drug delivery and cryopreservation technologies.
Optimizing adsorption efficiency: A novel application of SVM_Boosting_IGWO for methylene blue dye removal using low-cost fruit peels adsorbents
(Chemometrics and Intelligent Laboratory Systems, 2025-02-10) Nasma Bouchelkia , Hichem Tahraoui , Kheira Benazouz , Amal Mameri , Reguia Boudraa , Hamza Moussa , Nadia Hamri , Ryma Merdoud , Hayet Belkacemi , Abdelhalim Zoukel , Abdeltif Amrane , Mohammed Kebir , Lotfi Mouni
In this work, the potential for employing orange peels (OP) and potato peels (PP) as biosorbents to remove the methylene blue dye (MB) from aqueous solutions is studied. Several physicochemical methods, such as FTIR, SEM-EDX, X-ray diffraction, and pH point of zero charge measurement, were used to analyze the adsorbents. FTIR analysis revealed changes in peak intensities after dye adsorption. SEM analysis confirmed the presence of starch in the PP adsorbent, while no apparent pore structure was observed in the OP adsorbent. EDX analysis showed that carbon and oxygen were the main components on the surfaces of OP and PP. X-ray diffraction patterns indicated that both adsorbents were amorphous materials. The impact of different factors, including adsorbent dosage, contact time, temperature, initial dye concentration, pH and particle size, on the biosorption process was studied. Kinetic studies revealed that equilibrium was reached within a few minutes of contact, and the MB removal followed the pseudo-second-order model.Furthermore, a novel predictive model combining Support Vector Machine (SVM) with Boosting and the Improved Grey Wolf Optimizer (IGWO) algorithm was developed. The SVM-IGWO-Boosting model exhibited excellent performance in predicting methylene blue adsorption, demonstrating perfect correlation and low prediction error. The IGWO optimization approach effectively optimized the input parameters for the adsorbents, resulting in excellent agreement between exper imental and predicted values. Moreover, OP showed higher efficiency in removing methylene blue compared to PP, with a maximum capacity of 111.75 mg/g for OP and 96.67 mg/g for PP using IGWO. The use of orange peels and potato peels as agricultural waste for methylene blue removal offers an efficient and sustainable solution. The SVM-IGWO-Boosting predictive model, in conjunction with the IGWO optimization approach, provides a promising tool for predicting and optimizing the adsorption efficiency of MB adsorbents. These findings present valuable prospects for real-world applications requiring accurate and reliable predictions.
Prevalence of childhood overweight – obesity and associated factors: A school‑based, cross‑sectional study northern Algeria
(Nutrire, 2025-05-22) Salim Zaabar · Mohammed Khalil Mellal · Mustapha Aourir · Belkacem Zennache · Sofiane Boudalia · Zoubeida Meghlaoui · Lynda Messaoudene · Yasmine Brahimi · Nassim Madi · Khokha Mouhoubi · Amina Abbou · Lamia Medouni · Zahia Brara · Sabiha Bechir · Khodir Madani
Abstract Purpose To determine the prevalence of overweight/obesity and stunting among primary schoolchildren, and the associated factors. Methods This is a cross-sectional study conducted from February to September 2023 across 577 primary schools in Bejaia Province, Northern Algeria. Schoolchildren’s anthropometric measurements (weight and height) were obtained through direct physical assessments and were subsequently used to classify their weight and growth status according to WHO growth refer ence standards. Dietary habits school environment, and physical activity levels were obtained using survey questionnaires. Multivariate logistic regression analyses were performed to assess the associations between overweight/obesity and factors including dietary habits, physical activity, and the school environment. Results Overall, 446 (77.3%) primary schools participated in the study yielding a sample of 14,283 schoolchildren aged 9–13 years. Boys had a higher prevalence of obesity and severe obesity compared to girls. Overweight prevalence peaked at age 10 in girls (23.11%, 95% CI: 21.42–24.85%) while obesity peaked in boys at age 9 (18.80%, 95% CI: 11.14–28.76%). Obesity was strongly associated with stunting, particularly in boys (aOR=5.78, 95% CI: 4.41–7.66, p<0.001). Engagement in sportive activities was associated with lower overweight/obesity prevalence, while frequent snacking showed positive associations. Overweight and obesity were more prevalent in urban and suburban schools compared to rural settings. Conclusion This study reveals concerning patterns of childhood overweight/obesity and stunting in northern Algeria, high lighting the urgent need for targeted public health interventions addressing nutritional education and physical activity promotion.
Assessment of the Antibacterial Potential and Safety Profile of Lactic Acid Bacteria Strains Isolated from Algerian Raw Milk
(Asian journal of Dairy and food Research, 2025-05-22) Mohamed Amine Hammadeche , Nassim Madi , Karima Aoues , Amel Kaced , Amel Doumandji
Background: Raw milk harbors most lactic acid bacteria. Research in this field is a hot topic with the aim of using them for probiotic and/or technological applications. Methods: Lactic acid bacteria were isolated on MRS and M17 media. Antibacterial activity was tested by the direct method based on spots against four food-borne pathogens. Isolates demonstrating high antibiosis activity were selected following a statistical analysis (Two-way-ANOVA) and identified by MALDI-TOF MS. The safety profile was assessed by sensitivity to antibiotics, hemolytic activity, the ability to produce gelatinase and DNase and finally, the aptitude of lactic strains to form biogenic amines. Result: A total of 172 strains were isolated, of which 103 were presumed to be lactic acid bacteria. The results of the antibacterial activity demonstrated that 82 strains exerted an antagonistic effect against all reference strains and 12 of these showed significant antibiosis activity. Selected strains were identified as Lactococcus lactis ssp lactis (50%) and Enterococcus faecium (50%). All strains of Lactococcus lactis ssp lactis (LMMDR33, LMMDR06, LMMDR22, LMMDR26, LMMDR05 and LMMDR23) and strains LMMDR27, LMMDR16 and LVBBR13 of Enterococcus faecium demonstrated a good safety profile. They were retained for valorization
A Comprehensive Review of Pesticide Elimination Methods from Fruits and Vegetables Over the Past Two Decades: Optimizing Produce Safety for Sustainable Food Systems
(Journal of Research and Innovation in Food Science and Technology, 2025-04-23) Zoubeida Meghlaoui , Hocine Remini , Yasmine Remini-Sahraoui , Mohamed Khalil Mellal , Sofiane Boudalia , Yasmine Brahimi , Samira Negrichi , Ayoub Allam , Lamia Medouni-Haroune , Lynda Messaoudene , Amina Abbou , Amokrane Mahdeb , Nassim Brahimi , Meriem Amrane-Abider , Zahia Brara , Khodir Madani
The increasing use of pesticides in agriculture, valued at approximately 43.2 billion USD, has raised significant concerns regarding food safety and human health. This study reviews the effectiveness of various pesticide residue removal methods applied to fruits and vegeta bles (F & V). A total of 57 studies published between 2005 and 2022 were analyzed, cate gorizing the methods into 28 household techniques, 19 advanced methods, and 10 com bined approaches. Household methods, such as washing under running water, achieved re moval rates of up to 90%, while peeling ensured complete (100%) elimination of residues. The addition of salt or vinegar solutions improved removal efficiency, reaching 92%. Ad vanced methods, notably ozonation, demonstrated high efficacy with up to 95% removal. The most effective approaches were combined techniques, integrating washing, ultrasound, and ozonation, which achieved residue elimination rates of up to 99%. Despite their effi ciency, advanced methods face limitations due to high costs and technological constraints, reducing their accessibility for widespread use. This review underscores the necessity of an integrated approach to enhance food safety. Additionally, it highlights the need for further research on the long-term impact of these removal methods on the nutritional quality of F & V. These findings provide essential insights for consumers, farmers, and the food industry, contributing to the development of more effective and practical food safety strategies.
RSM and ANN modeling-based optimization approach for the development of alginate-chitosan particles containing Rosmarinus officinalis essential oil
(Polymer Bulletin, 2025-03-22) Lamia Taouzinet · Sofiane Fatmi · Amina Kribeche · Mohamed Skiba · Mokrane Iguer-Ouada
Alginate is a multifunctional biopolymer employed in diverse applications, particu larly in delivery systems that safeguard bioactives in acidic environments. Chitosan demonstrates various functionalities, including enhanced mucoadhesion and the facilitation of controlled release in alkaline conditions. Current research focuses on encapsulating essential oils in alginate and chitosan. The study aimed to encapsulate Rosmarinus officinalis essential oil within the alginate-chitosan sphere to enhance its physical properties and investigate the protective effects of alginate-chitosan Ros marinus essential oil particles during sperm cryopreservation. The effects of fac tors influencing cell motility were optimized and modeled using response surface methodology and artificial neural networks. Results indicated a high goodness of fit for the artificial neural network model with an R2 of 0.999, compared to 0.991 for the response surface methodology. The optimal solution involved 2 g of alginate, 1.05 g of chitosan, and 80 μl of Rosmarinus essential oil. The optimal formulation was characterized by assessing drug entrapment efficiency, drug-excipient interac tion, swelling index, and lipid peroxidation state. At pH 6.8, alginate-chitosan-Ros marinus essential oil particles exhibited a significant swelling index. These findings suggest that the formation of alginate-chitosan through cross-linking holds promise for solubilizing and delivering Rosmarinus essential oil to sperm under cryopreser vation conditions. Adding alginate-chitosan-Rosmarinus essential oil complex to the extender enhances its ability to remove free radicals and thus protects sperm during the cryopreservation process. A methodological approach utilizing either response surface methodology or artificial neural networks can establish alginate-chitosan encapsulation effectively. Importantly, the artificial neural network model outper formed response surface methodology in prediction and optimization accuracy.
Cellulose nanocrystals (CNC) from Diss plant: bleaching treatment effect
(International Journal of Materials Research, 2025-04-02) Lilia Benchikh, Yazid Ait Ferhat, Melia Guessoum, Abdelhafid Merzouki and Yves Grohens
Abstract: In this work, we investigate the efficiency of different pretreatment methods and their impact on the characteristics of cellulose nanocrystals (CNC) extracted from the Diss plant, a widely available plant in the North African region. Two extraction methodologies were employed, focusing on the effects of bleaching treatments using hydrogen peroxide and sodium chlorite. Fourier transform infrared spectroscopy confirmed the effective removal of non-cellulosic materials, yielding CNCs with a cellulose I crystalline structure. The Zeta potential analysis revealed an increased presence of hydroxyl groups on the CNC surface. Scanning electron microscopy showed that CNCs tend to form agglomerates upon drying. Thermogra vimetric analysis demonstrated that the CNCs exhibit considerable thermal stability, with degradation tempera tures ranging from 100 °C to 350 °C.
In Vitro and In Silico Assessment of the Probiotic and Technological Potential of Lacticaseibacillus rhamnosus MW019593 Isolated from Algerian Cow’s Milk
(Probiotics and Antimicrobial Proteins, 2025-03-13) Nassim Madi · Amine M. Boukerb · Loubna Mekdade · Assia Ikhlef · Asma Meghezzi · Abderrahmane Selmania · Nathalie Connil
This study evaluated the in vitro and in silico probiotic potential and technological characteristics of Lacticaseibacillus rhamnosus MW019593 isolated from Algerian cow’s milk. Safety was verified by the absence of hemolytic activity, biogenic amine production, and antibiotic sensitivity. Functional probiotic properties of L. rhamnosus MW019593 included moderate inhibition of gastrointestinal pathogens such as Salmonella, Listeria, Clostridioides, and Escherichia coli, tolerance to pH 2–3 and up to 1% bile salts, 46.4% hydrophobicity, 42.4% autoaggregation, and 12.9% adhesion to Caco-2/TC7 cells with out cytotoxicity. The ability to form biofilms with a thickness of 23.7 µm, comparable to L. rhamnosus ATCC 53103, was observed using confocal microscopy. Technologically, L. rhamnosus MW019593 and ATCC 53103 showed similar growth profiles and biomass yields, reaching approximately 5× 109 CFU/mL with a 63% yield after lyophilization. No significant difference in strain viability was noted at 4 °C and−20 °C, which are suitable temperatures to maintain their viability for 3 months. Whole genome sequencing (WGS) was conducted on L. rhamnosus MW019593, followed by comprehensive in silico genomic analysis. Taxonomic validation using average nucleotide identity (ANI) and genome-to-genome distance hybridization (GGDH) confirmed the strain’s affiliation to this species. Gene screening revealed the absence of virulence and antibiotic resistance genes, while beneficial genes, including those for bacteriocin production, were identified. These findings, supported by the demonstrated safety, functional, and technological properties of the strain, highlight its promising potential for food and therapeutic applications, pending in vivo validation.
Characterization of Chemlal Olive pomace and its extracted oil: chemical composition, quality indices, and FTIR analysis
(Journal of measurment and charactérization, 2025-09-23) Lynda Guerboub · Ouahiba Soufi-Maddi · Linda Ouldsaadi · Lamia Medouni-Haroune · Mostapha Bachir-bey · Khodir Madani · Lila Boulekbache-Makhlouf
Abstract The reuse and valorization of solid by-products from olive oil mills are attracting growing interest in the food, pharmaceu tical, and cosmetics sectors due to the recovery of vital bioactive compounds and residual oil, which can be incorporated into functional foods, cosmetics, and pharmaceuticals products. This study investigates, for the first time, the chemical composition, biological activities, and potential valorization of Chemlal olive pomace from Algeria, both fresh and dried. The pomace was showed standard compliant values for moisture, acidity, and pH, a high phenolic content (50.56 g GAE/ kg before drying and 63.53 g GAE/kg after drying), with significant antioxidant activity (DPPH• inhibition of 78.34% before drying and 89.53% after drying). Fourier-transform infrared (FTIR) spectroscopy confirmed the presence of func tional groups associated with phenolic compounds, triglycerides, and fatty acids, indicating strong potential for functional compounds recovery. The extracted oil had slightly elevated acidity and dominant oleic acid content (64.60%), consistent with conventional olive oil profiles. Notably, the oil also exhibited good antioxidant activity (87.43%), and a measurable phenolic content (30.98 g GAE/kg). These findings contribute to the existing body of knowledge on olive pomace and highlight its potential as a valuable resource for the agri-food and pharmaceutical industries. They also emphasize the importance of drying as a crucial pre-treatment for stabilization and optimal valorization.
Optimization of the Microwave-Assisted Extraction of Total Phenolic Compounds (TPCs) From Almond Skins Through Artificial Neural Networks (ANNs) and Assessment of the Antioxidant and Antihyperglycemic Activity of the Extracts
(Food Processing and Preservation, 2025) Lila Harkat-Madouri, Hanane Touati, Lila Boulekbache-Makhlouf, Khodir Madani, and Hayate Haddadi-Guemghar
Optimization by response surface methodology (RSM) and artificial neural networks (ANNs) was efficaciously applied to study the operating parameters of microwave-assisted extraction (MAE) in the recovery of total phenolic compounds (TPCs) from almond skins. These models were used to evaluate the effects of process variables and their interaction towards the attainment of their optimum conditions. A comparison of statistical parameters showed that ANN was more consistent (R2 = 0 99) than RSM (R2 = 0 97) to predict a TPC by MAE. Therefore, the following conditions were proposed: microwave power of 562W, extraction time of 30 s, and ethanol concentration of 53%, corresponding to an optimal TPC yield of 560.79 mg gallic acid equivalents (GAEs)/ 100 g of dry weight (DW). The almond skin extract exhibited a high antioxidant activity tested by 1,1-diphenyl-picrylhydrazyl (DPPH) radical scavenging activity (IC50 = 5 39 ± 0 35 μg/mL), phosphomolybdate ammonium essay, hydroxyl radical scavenging activity (IC50042002mg/mL), and ability of chelating ferrous ions. The in vitro antihyperglycemic activity test revealed that the almond skin extract inhibits strongly α-amylase activity with IC50 = 27 87 μg/mL which was close to IC50 of the therapeutic drug acarbose (IC50 = 14 24 μg/mL).
Study of physical activity in obese adolescents in Algeria
(Archive de pediatrie, 2025-02) Fouzia Yous , Salim Zaabar , Omar Aoun , Nawel Adjeroud-Abdellatif , Radia Arab , Khodir Madani
Simultaneous Determination of Acrylamide, Caffeic Acid, and Caffeine in Commercial Coffee Capsules Using a Rapid and Validated HPLC-DAD Approach
(The north african journal of food and nutrition research, 2025-12-09) Wassila Belkhiri-Beder , Lamia Taouzinet, Zahra Toutou , Khodir Madani , Sofiane Fatmi
Background: Coffee capsules are increasingly consumed worldwide and represent a complex source of bioactive compounds, notably caffeine (stimulant), caffeic acid (antioxidant), and acrylamide (toxicant). Aims: The objectives of this investigation were twofold: (i) assess total phenolic content (TPC), antioxidant capacity, pH, and browning index, across seven commercial capsule products (comprising six Algerian brands widely consumed locally and one European comparator with undisclosed blend/roast); and (ii) to develop and validate an HPLC – Diode Array Detection (DAD) procedure for the simultaneous determination of caffeine, caffeic acid, and acrylamide , serving as joint indicators of product quality and safety. Methods: The TPC was determined employing the Folin–Ciocalteu method, while antioxidant activity was assessed by the DPPH radical scavenging assay. The simultaneous quantification of caffeine, caffeic acid, and acrylamide were performed by a validated HPLC – DAD procedure in accordance with International Council for Harmonization (ICH) guidelines. Results: The resulting coffee brews exhibited pH values ranging from 5.64 to 6.25 and browning indices between 0.280 and 0.482, reflecting roasting differences. Despite a modest TPC (0.9 to 2 mg GAE/g), the antioxidant activity was high, (up to 95.3% inhibition DPPH). Caffeine was consistently detected (0.05 – 0.33 mg/25 mL), confirming its stimulant role. Caffeic acid was present in lower concentrations (0.001 – 0.14 mg/25 mL), contributed to the antioxidant potential. In contrast, acrylamide was detected in all analyzed samples ranging from 4.75 to 28.15 µg/25 mL). On a dry coffee basis, these concentrations correspond to 458 – 4023 µg/kg. A majority of the capsules yielded levels that exceed the European Commission’s benchmark of 400 µg/kg for roasted coffee, thereby underscoring consumer-relevant exposure level and raising toxicological concern. Conclusions: Coffee capsule brews demonstrated notable antioxidant capacity despite modest TPC, with caffeine ubiquitous and caffeic acid detectable. The presence of acrylamide was confirmed in all samples at levels that warrant monitoring. The validated HPLC–DAD workflow enables the simultaneous determination of these markers and offers a robust basis for integrated quality control and risk assessment in encapsulated coffee products.
Stevia and Steviol Glycosides in Dairy Products: An Overview on Usage, Microbial Dynamics, and Gut Health Effects – a Review Article
(Polish Journal of Food and Nutrition Sciences, 2025-12-12) Loubna Mekdade , Nassim Madi , Hossem Sahraoui
Stevia rebaudiana (Bertoni), a plant belonging to the Asteraceae family, has long been valued as a natural alternative to sugar. The sweet compounds it contains, known as steviol glycosides, can be up to 450 times sweeter than sucrose. In recent years, stevia has taken a prominent place among natural additives, as rising interest in natural products continues to emphasize plant-based alternatives. This paper focused on the potential benefits of incorporating stevia into dairy products. It provides a general overview of the use of stevia as a sugar substitute, mainly recognized for its zero-calorie sweetening properties. Moreover, it highlights that the whole leaf of S. rebaudiana contains additional bioactive compounds, including minerals, vita mins, and antioxidants, which may contribute to its nutritional value. This study also highlights the effects of adding stevia to dairy products, both as a prebiotic that enhances the viability of lactic acid bacteria and as an additive that inhibits the growth of spoilage-causing bacteria such as Escherichia coli, Salmonella enterica subsp. enterica serovar Typhi, and Staphylococcus aureus. While contributing to intestinal well-being by promoting the growth of gut microbiota, the addition of stevia to dairy products improves their sensory properties. In dairy systems, the incorporation of stevia hay into livestock feed may also be explored as a strategy to improve milk yield and quality. Given the growing trend in the consumption of probiotic and prebiotic foods, incorporating functional ingredients such as stevia into dairy products appears increasingly relevant.
Development and validation of a routine UV spectroscopic method for determination of camptothecin in cyclodextrin complexes, liposomes, niosomes, and solid dispersions in PEG 6000
(Biopolymer Applications Journal, 2025-07-04) Sofiane FATMI , Wassila BELKHIRI-BEDER , Hayet Ahlem LEZRAG , Zahra TOUTOU , Lamia TAOUZINET , Malika LAHIANI-SKIBA , Mohamed SKIBA and Mokrane IGUEROUADA
Abstract A robust UV–Vis spectroscopic assay was developed and validated for quantifying camptothecin (CPT) in complex drug carriers, including cyclodextrin inclusion complexes, liposomal and niosomes suspensions, and PEG 6000 solid dispersions, addressing persistent analytical challenges posed by CPT’s poor solubility and labile lactone ring. The method exhibited exceptional linearity over 1–50 µg·mL⁻¹ (y = 0.0198 x + 0.0032, R² = 0.9991), specificity with no interference from excipients (102.17 % recovery; RSD = 0.85 %), and accuracy/precision meeting ICH Q2 criteria (mean recovery = 99.96 %; RSD < 2 %). The limit of detection (0.5 µg·mL⁻¹) and quantification (1.5 µg·mL⁻¹) enabled rapid (< 5 min) throughput without the need for costly HPLC columns or organic solvents, substantially reducing per-sample cost and environmental burden. This UV method not only surpasses many chromatographic techniques in speed and simplicity but also preserves the CPT lactone form by minimising exposure to aqueous neutral pH, thereby providing a reliable analytical tool for routine industrial quality control of advanced CPT formulations.
Effect of sonication on microwave inactivation of Escherichia coli in an orange juice beverage
(Journal of Food Process Engineering-Wiley, 2021-02-21) Ourdia-Nouara Kernou, Amine Belbahi, Akila Amir, Kenza Bedjaoui, Kamelia Kerdouche, Sofiane Dairi, Omar Aoun, Khodir Madani
The survival of Escherichia coli ATCC 25922 in orange juice treated with microwave and/or ultrasound was evaluated; Weibull model was fitted to survival curves to describe inactivation kinetics; and the effect of combined microwave-ultrasound treatments was assessed. Ultrasonic treatment (42 kHz) has no significant effect on the survival rate reduction (1.3 log for 60 min of sonication) of E. coli. However, sonication can increase the efficiency of microwave inactivation. The use of ultrasound as pre-treatment was more effective than when used in post-treatment, that is, a reduction of 8.0 log was achieved by a combination of ultrasound (20 min) followed by a microwave (900 W/30 s), while 4.0 log was obtained when these both processes were reversed. The scale parameter α estimated from microwave-inactivation kinetics combined with an ultrasound pre-treatment was found to be lower than those obtained with a post-treatment, which, in turn, are lower than those estimated for microwave or ultrasound alone. These results demonstrate the effectiveness of ultrasound as a pre-treatment to improve the inactivation of E. coli by microwave processing in orange juice beverages.
Ethnobotanical survey of three members of family Lamiaceae among the inhabitants of Bejaia, Northern Algeria
(Journal of Complementary and Integrative Medicine- De Gruyter, 2020-12-25) Nabyla Khaled-Khodja, Fatiha Brahmi, Khodir Madani, Lila Boulekbache-Makhlouf
Times New Roman (Titres CS)
Dairy dessert processing: Effect of sugar substitution by date syrup and powder on its quality characteristics
(Journal of Food Processing and Preservation- Wiley, 2020-02-24) Kahina Djaoud, Lila Boulekbache-Makhlouf, Massinissa Yahia, Hafid Mansouri, Nassima Mansouri, Khodir Madani, Alberto Romero
Second-grade dates can be valorized by its uses in the production of nutritive foods with health benefits. Date syrup (DS), date powder (DP), and their different concentrations, using D-optimal mixture design, were used for the formulation of dairy desserts: syrup dairy dessert (SDD) and mixture dairy dessert (MDD). The optimized mixture proportions of the best dairy dessert formulation, containing date syrup (14%) and powder (2%) as a sugar substitute (0%), were determined to achieve the desired sensory for the effective formulation process. Significant differences in water, protein, fat, and sugar contents were found between SDD, MDD, and commercial dairy dessert (CDD) used as standard, respectively. SDD exhibited the highest total phenolic content (0.65 ± 0.04 mg GAE/g), DPPH inhibition (4.00 ± 0.82%), and reducing power (2.75 ± 0.10%) followed by MDD and CDD. The absence of germs indicated a high microbiological quality of dairy desserts. Rheological measurements revealed that all samples exhibited a weak gel structure and shear thinning behavior. Desserts supplemented with dates led to desserts with an homogeneous structure
Spatiotemporal trend analysis of runoff and actual evapotranspiration in Northern Algeria between 1901 and 2020
(Modeling Earth Systems and Environment, 2022-07-09) Amir Aieb, Ismahen Kadri, Khalef Lefsih, Khodir Madani
In water resources management, the estimation of the water balance component is mainly used to control dams, agriculture, and irrigation developments and also to provide water supply for drinking and industry. The spatial trend analysis applied to the Northern Algerian region to determine the significant changes in rainfall–runoff (IARR) and actual evapotranspiration (IAEa) required a sufficient period. In this study, we carried out this analysis in four periods between 1901 and 2020, according to the data availability of climate variables (IAR, and IAT), since the hydrological measurement posed a big problem during this period. In this regard, a set of nonparametric and empiric water balance models were applied and compared on five bioclimatic floors of Northern Algeria between 1991 and 2020 to estimate IARR and IAEa between 1901 and 1990. The results obtained from several tests, such as regression analysis, scattergram graphs, R2, R2Adj, MSE, RMSE, and DW, proved that Yang's model gives the best performance and good results in very humid regions when replacing n by 2. On the other hand, Schreiber and Pike’s models are more efficient in the humid and semi-humid regions, respectively. Furthermore, the model proposed by Sharif is well performed in the Mediterranean and semi-dry areas. The variability analysis given by the t test and the regression curves is applied on IAEa and IARR in each bioclimatic floor during intervals measurement of [1901, 1930], [1931, 1960], [1961, 1990], and [1991, 2020]. It showed a significant change in both components between 1961 and 1990 in very humid, semi-humid, and Mediterranean regions when compared with observations done between 1931 and 1960. Moreover, a large variation and significant trend in IARR were also observed in humid and semi-humid areas between 1991 and 2020 compared to the previous periods.
Comparison of chemical composition and biological activities of Algerian seed oils of Pistacia lentiscus L., Opuntia ficus indica (L.) mill. and Argania spinosa L. Skeels
(Industrial Crops and Products-ELSEVIER, 2020-04-28) Fatiha Brahmi, Souhila Haddad, Kenza Bouamara, Drifa Yalaoui-Guellal, Emmanuelle Prost-Camus, Jean-Paul Pais de Barros, Michel Prost, Atanas G. Atanasov, Khodir Madani, Lila Boulekbache-Makhlouf, Gérard Lizard
Many parameters can influence the chemical profiles and the biological activities of seed oils. It was therefore of interest to study Algerian seed oils, whose caharacteristics are not well known. So, the physicochemical properties and nutrient profiles (fatty acids, phytosterols, polyphenols) of seed oils from Pistacia lentiscus L. (PL), Opuntia ficus-indica (L.) mill. (OFI), and Argania spinosa L. Skeels (AS) were determined. The antioxidant and antimicrobial activities of the oils were also characterized. The physicochemical parameters of the oils are closely related to the standard values. PL oil is distinguished by its high content of pigments. AS and OFI oils were dominated by linoleic acid, at 39.1 ± 0.5 and 55.8 ± 0.6%, respectively, while the oleic acid (41.2 ± 0.4%) was the major fatty acid in the oil of PL. The analysis of phytosterol levels showed that β-sitosterol was present in high amounts in the three oils, of 387.44 ± 3.04, 87.92 ± 0.72 and 58.79 ± 1.19 mg/100 g of oil in OFI, AS and PL oils, respectively. The characterization of phenolics revealed only the presence of protocatechuic acid in the PL oil and p-coumaric and t-cinnamic acids in AS oil. The antioxidant activity was evaluated by using the phosphomolybdate assay and the scavenger activity of the DPPH• radical. PL and OFI oils showed the highest antioxidant capacity compared with AS. Very weak antibacterial and antifungal effects, evaluated against four bacterial and six fungal strains, were found. Given the chemical characteristics and antioxidant properties of Algerian PL, OFI and AS seed oils, our results highlight the potential benefit of these oils for human health.
Phenolic compounds from Nerium oleander leaves microwave assisted extraction, characterization, antiproliferative and cytotoxic activities
(food & function- Royal Society of Chemistry, 2020-06-20) Siham Ayouaz, Sheila Cristina Oliveira-Alves, Khalef Lefsih, Ana Teresa Serra, Andreia Bento Da Silva b d, Madani Samah f, Jakub Karczewski g, Khodir Madani h, Maria Rosário Bronzeb
A microwave-assisted extraction (MAE) method was used for the extraction of phenolic compounds from Nerium oleander leaves. The influence of variables such as ethanol concentration, microwave power, irradiation time and liquid/solid ratio on polyphenol extraction was modelled using a second-order regression equation based on response surface methodology (RSM). The optimal conditions for MAE were: extraction solvent 35% ethanol concentration, 500 W microwave power, 60 s irradiation time and a solvent/material ratio of 20 mL g−1. Under optimal MAE conditions, the recovery of TPC was 25.752 mg GAE per g dw. 19 compounds have been identified by HPLC-ESI-MS/MS analysis; the main compounds identified were chlorogenic acid, rutin and quinic acid esters, such as caffeoylquinic acids and dicaffeoylquinic acids. Additionally, the optimized extract demonstrated potential to inhibit HT29 colorectal cancer cell growth (EC50 = 2.432 μg mL−1) without presenting cytotoxicity in confluent Caco-2 cells, a model of human intestinal epithelium. These results supply new information about the phenolic composition of Nerium oleander leaves and their antiproliferative effect.

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