In vitro Antioxidant and Anti-Inflammatory Effects of the Hydro-Methanolic Extract of Phlomis crinita from North Algeria

Abstract

Phlomis crinita is a medicinal plant commonly used in Algerian folk medicine as a remedy for ulcers , haemorrhoids and healing of wounds. The current study aimed to evaluate the anti - inflammatory and antioxidant effects of this plant and to provide a scientific basis for its use. Protein denaturation and membrane stabilization assays were used to assess the anti - infla mmatory action in vitro . DPPH free radical - scavenging and reducing power tests were used to determine the antioxidant activity. The plant methanol extract exhibited potential anti - inflammatory activity , demonstrating its ability to prevent protein denatura tion and stabilize the membrane of human red blood cells. It had IC 50 values of 143.64 ± 2.94 and 119.63 ± 0.80 μg/mL, respectively, compared to d iclofenac sodium (IC 50 value of 60.60 ± 2.55 and 53.47 ± 0.93 μg/mL , respectively). P. crinita showed strong antioxidant DPPH scavenging activity ( IC 50 = 82.07 ±0.37 μg/mL ) compared to a scorbic acid (62.48 ±0.56) and exhibited a strong reducing potential. The results suggested that the plant might be a potential candidate for oxidative stress prevention and inflammatory disease treatment .