THE PRESENCE OF VITAMIN C ENHANCES THE PROTECTION OF CHOLESTEROL-CYCLODEXTRIN AND VITAMIN E-CYCLODEXTRIN IN CRYOPRESERVATION OF BULL SEMEN

Abstract

Abstract BACKGROUND: Cryopreservation of sperm cells is a well-established technique. However, cryopreserved sperm cells can have significant cellular damage, primarily due to oxidative stress. OBJECTIVE: To investigate the synergistic effects of vitamin C with vitamin E-loaded cyclodextrins on the quality of cryopreserved bull sperm cells in combination with cholesterol-loaded cyclodextrins. MATERIALS AND METHODS: The ejaculates from nine mature bulls were divided into six equal aliquots for different treatments, including vitamin C (VitC), vitamin E loaded cyclodextrin (CD-VitE), CD-VitE+VitC, cholesterol loaded cyclodextrin (CD-CHL), CD-CHL + CD-VitE + VitC and the control. The sperm motility (CASA), membrane integrity (HOST) and lipid peroxidation (TBARS) were assessed after freezing and thawing. RESULTS: The combination of CD-CHL + CD-VitE + VitC retained the highest sperm motility and membrane integrity, as well as resulted in the least lipid peroxidation. CONCLUSION: The study shows the synergistic effects of vitamin C, vitamin E and cholesterol in improving the cryopreservation outcomes of bull sperm.