Keltoum Bouziane-Ait BessaiNabila Brahmi-ChendouhFatiha BrahmiSofiane DairiKhokha MouhoubiFerriel KermicheKenza BedjaouiKhodir MadaniLila Boulekbache-Makhlouf2026-07-232026-07-232023-07-122092-6456https://dspace.crtaa.dz/handle/123456789/88The effect of different storage methods (ambient temperature (A), refrigeration at 4 °C (R) and freezing at − 18 °C (F)), on the phytochemistry of an Algerian spice (paprika powder), was assessed. The optimized extract was obtained under the optimum conditions of ultrasound-assisted extraction (UAE) using response surface methodology (RSM) coupled with a Box–Behnken Design (BBD). This extract was evaluated for its total phenolics content (TPC), total flavonoids content (TFC) and its anti- oxidant and antibacterial activities. Under the optimum conditions (5 min for the irradiation time, 40% for the amplitude, 80% for ethanol concentration and 50% for solid–liquid ratio) the TPC was 12.23 ± 1.01 mg Gallic Acid Equivalent/gram of Dried Powder (mg GAE/g DP) which is very close with experimental assay. The TPC are better preserved at A whereas TFC and the antioxidant activity at F, and the antibacterial activity depend on the storage methods and the strains tested.enPaprikaStoragePhenolic compoundsUltrasound-assisted extractionBox–Behnken designBiological activitiesArticle