Allaeddine KHELLOUF, Amine BENBERKAN, Lamia TAOUZINE, Sofiane FATM and Mokrane IGUER-OUADA2026-07-272026-07-272025ISSN: 1742-0644 ISSN-L: 0143-2044https://dspace.crtaa.dz/handle/123456789/135Abstract BACKGROUND: Cryopreservation of sperm cells is a well-established technique. However, cryopreserved sperm cells can have significant cellular damage, primarily due to oxidative stress. OBJECTIVE: To investigate the synergistic effects of vitamin C with vitamin E-loaded cyclodextrins on the quality of cryopreserved bull sperm cells in combination with cholesterol-loaded cyclodextrins. MATERIALS AND METHODS: The ejaculates from nine mature bulls were divided into six equal aliquots for different treatments, including vitamin C (VitC), vitamin E loaded cyclodextrin (CD-VitE), CD-VitE+VitC, cholesterol loaded cyclodextrin (CD-CHL), CD-CHL + CD-VitE + VitC and the control. The sperm motility (CASA), membrane integrity (HOST) and lipid peroxidation (TBARS) were assessed after freezing and thawing. RESULTS: The combination of CD-CHL + CD-VitE + VitC retained the highest sperm motility and membrane integrity, as well as resulted in the least lipid peroxidation. CONCLUSION: The study shows the synergistic effects of vitamin C, vitamin E and cholesterol in improving the cryopreservation outcomes of bull sperm.enantioxidantbull spermcholesterolcryopreservationvitamin Cvitamin E.Article